Um nun aber Zellabstammungsbäume aus einem Zeitraffervideo zu extrahieren, müssen die Zellen segmentiert und verfolgt werden können. The online version of this article (doi:10.1186/s1285-x) contains supplementary material, which is available to authorized users.ĭynamische zelluläre Prozesse wie Zellzyklus, Signaltransduktion oder Transkription zu analysieren wird Live-cell-imaging mittels Zeitraffermikroskopie verwendet. Tracer allows multi-parametric morphological data to be visualized on object tracks, providing visualizations that have already been validated within the scientific community for time-lapse experiments, and combining them with simple graph-based measures for highlighting possible tracking artifacts.ĬellProfiler Tracer is a useful, free tool for inspection and quality control of object tracking data, available from. We present CellProfiler Tracer, a free and open-source tool that complements the object tracking functionality of the CellProfiler biological image analysis package. This makes quality assessment and algorithm adjustment a substantial challenge, particularly when dealing with hundreds of time-lapse movies collected in a high-throughput manner.
Cellprofiler nameandtypes error software#
Algorithms for cell tracking are widely available what researchers have been missing is a single open-source software package to visualize standard tracking output (from software like CellProfiler) in a way that allows convenient assessment of track quality, especially for researchers tuning tracking parameters for high-content time-lapse experiments. Time-lapse analysis of cellular images is an important and growing need in biology.
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